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免疫組織化学用 IgA 抗体試薬

免疫組織化学用 IgA 抗体試薬

IHC 抗体 - IgA



  • 陽性対照:

    Tonsil
  • 細胞局在化:

    Cytoplasm
  • 応用:

    IHC-P
  • 二次抗体:

    iVision™
  • 仕様/ml:

    1、3、6、0.2(Concentrated)

Product name

IHC Antibody --IgA


Packing specification

Code

Clone

Specifications

AR0847

TLR0847

0.1ml, 0.2ml, 1ml, 1.5ml, 3ml, 6ml, 11ml, 30ml

AR0515

SD410

0.1ml0.2ml, 1ml, 1.5ml3ml6ml11ml30ml

AR0516

SD411

0.1ml0.2ml, 1ml, 1.5ml3ml6ml11ml30ml

AP0138

polyclonal

0.1ml0.2ml, 1ml, 1.5ml3ml6ml11ml30ml


Intended use

For Research use only. IgA Antibody reagent is intended for use to qualitatively identify IgA by microscopy in sections of FFPE tissue using immunohistochemical detection system.


Principle

Immunoglobulin A (IgA) is a glycosylated protein of 160 kDa. It is produced as a monomer or as a J chain linked dimer. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. Add the primary antibody to bind the antigen on tissue sections, and then use HRP labeled secondary antibody binding primary antibody to form the secondary antibody-primary antibody-antigen complex. When DAB chromogenic solution is added, HRP reacts with enzyme substrate to produce brown insoluble reaction product, which indirectly indicating the existence of antigen. 


Main components

Immunoglobulin, antibody diluent


Storage

Store at 2~8℃ for 18 months


Sample requirements

FFPE tissues are usually cut into sections as thin as 35μm with a microtome. These sections are then mounted onto glass slides that are coated with a tissue adhesive.


Protocol

1. Sample preparationDeparaffinize the slides in xylene , , Ⅲ for 5 minutesTransfer the slides once through 100%, 100%, 95%, 75% alcohols for 2 minutes respectively. Rinse slides with deionized water for 30 seconds.

2BlockingBlock endogenous peroxidase activity by incubating sections in 3% H2O2 solution at room temperature for 5 minutes to block endogenous peroxidase activity. Rinse the slides with deionized water for 30 seconds.

3Antigen retrievalHeat the EDTA Antigen retrieval buffer to 100. Then place the slides in the boiled buffer and continue to heat for 1520 min. Naturally cool down for 30 minutes. Rinse the sample with wash buffer.

4Primary antibody incubation: Drain the slides. Add primary antibody to tissue, incubate at room temperature for 30 minutes. (use antibody diluent or PBS as control). Wash the slides in PBST for 2 times, 5 minutes for each time. If the Primary antibody is concentrated, please dilute it to RTU(ready to use) according to the information on packing. 

5. Secondary antibody: Drain the slides. Add secondary antibody to tissue and incubate at room temperature for 20 minutes. Wash the slides in PBST for 2 times, 5 minutes for each time.

6. DABDrain the slides. Add DAB to the tissue and incubate at room temperature for 5 min. Rinse slides with deionized water.

7. Hematoxylin stainingDrain the slides. Add Hematoxylin to the tissue and incubate at room temperature for 5 minutes. Rinse slides with water. Use the acid solution for differentiation. Rinse slides with water.

8. DehydrateDehydrate the slides in 75%, 95%, 100% alcohols for 2 minutes. Dry the slides. Cover stained tissue with a coverslip using mounting medium.


Positive localization

1. Positive localization: cytoplasm.

2. Positive control: tonsil.


Precautions

1. Please read the instruction carefully and become familiar with all components of the kit prior to use, Strictly follow the instruction during operation.

2. キットまたはキットのコンポーネントを使用した後には使用しないでください。

3. 訓練を受けた専門家のみがこのキットを使用できます。試薬を取り扱う際は、適切な白衣と使い捨て手袋を着用してください。

4. 皮膚、目、粘膜への化学物質の接触を避けてください。

5. 口でピペッティングしないでください。

6. 未使用の試薬、使用済みキット、廃棄物は、地域の規制に従って廃棄する必要があります。


メーカー_

会社名: アモイ タレント バイオメディカル テクノロジー株式会社

住所: 36100 中国、厦門市、海滄区、バイオメディカルパーク、2068 Wengjiao Road West、ビル B10、3 階と 4

電話: +86 592 6315755            

電子メール: tlsw@talentbimedical.com                

ウェブサイト: www.talentbimedical.com


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