免疫組織化学用コラーゲン IV 抗体試薬

【Product name】

IHC Antibody --Collagen Type IV

【Packing specification】

【Intended use】

For Research use only. Collagen Type IV Antibody reagent is intended for use to qualitatively identify Collagen Type IV by microscopy in sections of FFPE tissue using immunohistochemical detection system.

【Principle】

Collagen Type IV is a major component of the basement membrane and plays an important role in cell adhesion, migration, differentiation and growth. Add the primary antibody to bind the antigen on tissue sections, and then use HRP labeled secondary antibody binding primary antibody to form the secondary antibody-primary antibody-antigen complex. When DAB chromogenic solution is added, HRP reacts with enzyme substrate to produce brown insoluble reaction product, which indirectly indicating the existence of antigen. 

【Main components】

Immunoglobulin, antibody diluent

【Storage】

Store at 2～8℃ for 18 months。

【Sample requirements】

FFPE tissues are usually cut into sections as thin as 3～5μm with a microtome. These sections are then mounted onto glass slides that are coated with a tissue adhesive.

【Protocol】

1. Sample preparation：Deparaffinize the slides in xylene Ⅰ, Ⅱ, Ⅲ for 5 minutes；Transfer the slides once through 100%, 100%, 95%, 75% alcohols for 2 minutes respectively. Rinse slides with deionized water for 30 seconds.

2. Blocking：Block endogenous peroxidase activity by incubating sections in 3% H₂O₂ solution at room temperature for 5 minutes to block endogenous peroxidase activity. Rinse the slides with deionized water for 30 seconds.

3. Antigen retrieval：Heat the EDTA Antigen retrieval buffer to 100℃. Then place the slides in the boiled buffer and continue to heat for 15～20 min. Naturally cool down for 30 minutes. Rinse the sample with wash buffer.

4. Primary antibody incubation: Drain the slides. Add primary antibody to tissue, incubate at room temperature for 30 minutes. (use antibody diluent or PBS as control). Wash the slides in PBST for 2 times, 5 minutes for each time. If the Primary antibody is concentrated, please dilute it to RTU(ready to use) according to the information on packing. 

5. Secondary antibody: Drain the slides. Add secondary antibody to tissue and incubate at room temperature for 20 minutes. Wash the slides in PBST for 2 times, 5 minutes for each time.

6. DAB：Drain the slides. Add DAB to the tissue and incubate at room temperature for 5 min. Rinse slides with deionized water.

7. Hematoxylin staining：Drain the slides. Add Hematoxylin to the tissue and incubate at room temperature for 5 minutes. Rinse slides with water. Use the acid solution for differentiation. Rinse slides with water.

8. Dehydrate：Dehydrate the slides in 75%, 95%, 100% alcohols for 2 minutes. Dry the slides. Cover stained tissue with a coverslip using mounting medium.

【Positive localization】

1. Positive localization: Extracellular matrix.

2. Positive control: skin.

【Precautions】

1. Please read the instruction carefully and become familiar with all components of the kit prior to use, Strictly follow the instruction during operation.

2. キットまたはキットのコンポーネントを使用した後には使用しないでください。

3. 訓練を受けた専門家のみがこのキットを使用できます。試薬を取り扱う際は、適切な白衣と使い捨て手袋を着用してください。

4. 皮膚、目、粘膜への化学物質の接触を避けてください。

5. 口でピペッティングしないでください。

6. 未使用の試薬、使用済みキット、廃棄物は、地域の規制に従って廃棄する必要があります。

【メーカー】_

会社名: アモイ タレント バイオメディカル テクノロジー株式会社

住所: 36100 中国、厦門市、海滄区、バイオメディカルパーク、2068 Wengjiao Road West、ビル B10、3 階と 4階

電話: +86 592 6315755            

電子メール: tlsw@talentbimedical.com                

ウェブサイト: www.talentbimedical.com

【記号】