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免疫組織化学用 CD44 抗体試薬

免疫組織化学用 CD44 抗体試薬

IHC 抗体 -- CD44


  • 陽性対照:

    Tonsil
  • 細胞局在化:

    Cytoplasm/cell membrane
  • 応用:

    IHC-P
  • 二次抗体:

    iVision™
  • 仕様/ml:

    1、3、6、0.2(Concentrated)

商品名

IHC 抗体 -- CD44


梱包仕様

コード クローン

仕様

AM0049

DF1485

0.1ml 0.2ml 、1ml、 1.5ml 3ml 6ml 11ml 30ml

AR0496

SD391

0.1ml 0.2ml 、1ml、 1.5ml 3ml 6ml 11ml 30ml


使用目的

研究用途のみ。CD44 抗体試薬は、 免疫組織化学的検出システムを使用して、FFPE 組織切片の顕微鏡検査によってCD44 を定性的に識別するために使用することを目的としています


原則_

IN、LHRMIC4、CDW44、HCELL としても知られる CD44 は、細胞間相互作用、細胞接着および遊走に関与する細胞表面糖タンパク質です。 抗CD44は、尿路上皮における非腫瘍性変化から原位置尿路上皮癌を区別するのに有用である可能性がある。抗体を加えて組織切片上の抗原結合させ、次に一次抗体に結合するHRP標識二次抗体を使用して次抗体-一次抗体-抗原複合体を形成します   When DAB chromogenic solution is added, HRP reacts with enzyme substrate to produce brown insoluble reaction product, which indirectly indicating the existence of antigen. 


Main components

Immunoglobulin, antibody diluent


Storage

Store at 2~8℃ for 18 months


Sample requirements

FFPE tissues are usually cut into sections as thin as 35μm with a microtome. These sections are then mounted onto glass slides that are coated with a tissue adhesive.


Protocol

1. Sample preparationDeparaffinize the slides in xylene , , Ⅲ for 5 minutesTransfer the slides once through 100%, 100%, 95%, 75% alcohols for 2 minutes respectively. Rinse slides with deionized water for 30 seconds.

2BlockingBlock endogenous peroxidase activity by incubating sections in 3% H2O2 solution at room temperature for 5 minutes to block endogenous peroxidase activity. Rinse the slides with deionized water for 30 seconds.

3Antigen retrievalHeat the EDTA Antigen retrieval buffer to 100. Then place the slides in the boiled buffer and continue to heat for 1520 min. Naturally cool down for 30 minutes. Rinse the sample with wash buffer.

4Primary antibody incubation: Drain the slides. Add primary antibody to tissue, incubate at room temperature for 30 minutes. (use antibody diluent or PBS as control). Wash the slides in PBST for 2 times, 5 minutes for each time. If the Primary antibody is concentrated, please dilute it to RTU(ready to use) according to the information on packing. 

5. Secondary antibody: Drain the slides. Add secondary antibody to tissue and incubate at room temperature for 20 minutes. Wash the slides in PBST for 2 times, 5 minutes for each time.

6. DABDrain the slides. Add DAB to the tissue and incubate at room temperature for 5 min. Rinse slides with deionized water.

7. Hematoxylin stainingDrain the slides. Add Hematoxylin to the tissue and incubate at room temperature for 5 minutes. Rinse slides with water. Use the acid solution for differentiation. Rinse slides with water.

8. DehydrateDehydrate the slides in 75%, 95%, 100% alcohols for 2 minutes. Dry the slides. Cover stained tissue with a coverslip using mounting medium.


Positive localization

1. Positive localization: membrane.

2. Positive control: tonsil.


Precautions

1. Please read the instruction carefully and become familiar with all components of the kit prior to use, Strictly follow the instruction during operation.

2. DO NOT use the kit or any kit component after their.

3. Only trained professionals can use this kit. Please wear suitable lab coat and disposable gloves while handling the reagents.

4. Avoid contact of skin, eyes and mucous membranes with the chemicals.

5. DO NOT pipet by mouth.

6. Unused reagents, used kit and waste must be disposed according to local regulations.


Manufacturer

Company Name: Xiamen Talent Biomedical Technology Co.,Ltd

Address: No.3rd and 4th Floors , Building B10, No. 2068 Wengjiao Road West, BioMedical Park, Haicang District, Xiamen City, 36100 China

Tel: +86 592 6315755            

E-mail: tlsw@talentbiomedical.com                

Website: www.talentbiomedical.com


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